TDG Monoklonaler Antikörper

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• KD/KO Validated

TDG Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G2E2

Kat-Nr. : 66707-1-PBS

Synonyme

1G2E2, EC:3.2.2.29, G/T mismatch-specific thymine DNA glycosylase, hTDG, thymine DNA glycosylase

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  WB analysis using 66707-1-Ig (same clone as 66707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66707-1-Ig (TDG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66707-1-Ig (same clone as 66707-1-PBS)

  WB result of TDG antibody (66707-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDG transfected HEK-293 cells. This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66707-1-Ig (same clone as 66707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66707-1-Ig (TDG antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66707-1-Ig (same clone as 66707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66707-1-Ig (TDG antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66707-1-Ig (same clone as 66707-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66707-1-Ig (TDG antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66707-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66707-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TDG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TDG fusion protein Ag27408
• Vollständiger Name: thymine-DNA glycosylase
• Berechnetes Molekulargewicht: 410 aa, 46 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC037557
• Gene symbol: TDG
• Gene ID (NCBI): 6996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.