TDG Rekombinanter Antikörper

TDG Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240715E12

Kat-Nr. : 83780-3-PBS

Synonyme

240715E12, EC:3.2.2.29, G/T mismatch-specific thymine DNA glycosylase, hTDG, thymine DNA glycosylase

---

• 

  IF Staining of HepG2 using 83780-3-RR (same clone as 83780-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TDG antibody (83780-3-RR, Clone: 240715E12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83780-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83780-3-RR (same clone as 83780-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TDG Recombinant Antibody (83780-3-RR, Clone:240715E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 83780-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00744-1

  Cytometric bead array standard curve of MP00744-1, TDG Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83780-1-PBS. Detection antibody: 83780-3-PBS. Standard: Ag4190. Range: 1.25-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83780-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83780-3-RR against Human TDG were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

83780-3-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA TDG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TDG fusion protein Ag4190
• Vollständiger Name: thymine-DNA glycosylase
• Berechnetes Molekulargewicht: 410 aa, 46 kDa
• GenBank-Zugangsnummer: BC037557
• Gene symbol: TDG
• Gene ID (NCBI): 6996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.