TDO2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TDO2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Rezensionen (2)

Kat-Nr. : 15880-1-AP

Synonyme

TDO, TDO2, TO, TRPO, Tryptamin 2,3 dioxygenase, tryptophan 2,3 dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase

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Galerie der Validierungsdaten

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  WB analysis using 15880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15880-1-AP (TDO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15880-1-AP (TDO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15880-1-AP (TDO2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15880-1-AP (TDO2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15880-1-AP (TDO2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15880-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TDO2 antibody (15880-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 10 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Produktinformation

15880-1-AP bindet in WB, IHC, IF, FC, ELISA TDO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TDO2 fusion protein Ag8642
• Vollständiger Name: tryptophan 2,3-dioxygenase
• Berechnetes Molekulargewicht: 406 aa, 48 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC005355
• Gene symbol: TDO2
• Gene ID (NCBI): 6999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TDO2 (tryptophan 2,3-dioxygenase), IDO1 (indoleamine 2,3-dioxygenase 1) and IDO2 are three enzymes that catalyze the amino acid tryptophan into kynurenine in the kynurenine pathway (PMID: 23090118, 28469468). TDO2 plays an important role in neurological diseases such as Alzheimer's disease, Parkinson's disease and autism. Overexpression of TDO2 promoted tumor cell survival and was correlated with tumor grade and poor prognosis in triple negative breast cancer, brain tumors and esophageal squamous cell carcinoma. TDO2 may be a potential therapeutic target in some cancers. (PMID: 21976023, 30134247)

Publikationen

Species	Application	Title
human, mouse	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala ; KD Validated
human	WB	Nat Commun; Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization; Authors - Kadir Buyukcelebi
human,rat	WB,IHC,IF	Br J Pharmacol; Tryptophan 2,3-dioxygenase 2 plays a key role in regulating the activation of fibroblast-like synoviocytes in autoimmune arthritis.; Authors - Yan Chang ; KD Validated
mouse	WB	Front Immunol; DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota; Authors - Li-Ping Zhao
human	WB,IHC	Fertil Steril; Tryptophan catabolism is dysregulated in leiomyomas.; Authors - Tsai-Der Chuang
mouse	WB	J Med Chem; Discovery of 1-(Hetero)aryl-β-carboline Derivatives as IDO1/TDO Dual Inhibitors with Antidepressant Activity; Authors - Yu Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (03-10-2021)

works veryyy gooddd

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000 - 1/2000
• Cell Tissue Type: hepatoblastoma cells transduced with lentivirus expressing TDO2

FH

Hala (Verified Customer) (12-15-2020)

so efficient

• Applications: Western Blot
• Primary Antibody Dilution: 1/750 - 1/1000
• Cell Tissue Type: liver cells transduced with lentivirus expressing TDO2

TDO2 Antibody Western Blot validation (1/750 - 1/1000 dilution) in liver cells transduced with lentivirus expressing TDO2 (Cat no:15880-1-AP)