TDP-43 Monoklonaler Antikörper

TDP-43 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

1G4F5

Kat-Nr. : 66734-1-Ig

Synonyme

TARDBP, TDP 43, TDP43, 1G4F5, ALS10

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  WB analysis using 66734-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66734-1-Ig (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

66734-1-Ig bindet in WB, IHC, ELISA TDP-43 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001487
• Gene symbol: TDP-43
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

Publikationen

Species	Application	Title
human		Acta Neuropathol; Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology.; Authors - Alissa L Nana
mouse	WB	Int J Biol Sci; Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice.; Authors - Rui Zhang
mouse	IHC	Exp Neurol; Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions.; Authors - Nozomu Tawara
mouse		Sci Rep; A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin.; Authors - Phaneendra Chennampally
human		Nat Commun; TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression; Authors - Marta Garcia-Montojo
human	WB	J Nat Prod; Phenolics and Phenolic Glycosides from Wrightia pubescens and Their Hepatoprotective Activities; Authors - Xingxiang Chen