TDP-43 (N-terminal) Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TDP-43 (N-terminal) Rekombinant Antikörper für WB, IHC, IHC-Autostainer, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IHC-Autostainer, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

16A22

Kat-Nr. : 80002-1-RR

Synonyme

TDP-43 (for IF/FC), TARDBP, TDP 43, TDP43, TDP-43

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  WB analysis using 80002-1-RR

  Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 80002-1-RR

  HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 80002-1-RR

  WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

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  IP experiment of HAP1 using 80002-1-RR

  HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of HeLa using 80002-1-RR

  IP result of anti-TDP-43 (N-terminal) (IP:80002-1-RR, 4ug; Detection:80002-1-RR 1:20000) with HeLa cells lysate 1200 ug.

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  IHC staining of human brain using 80002-1-RR

  Immunohistochemical analysis of paraffin embedded human brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on Epredia 480S Autostainer.

• 

  IHC staining of human brain using 80002-1-RR

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer

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  IHC staining of mouse brain using 80002-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RREC80002 (TDP-43 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 80002-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TDP-43 (N-terminal) antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

• 

  IF Staining of HeLa using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HAP1 using 80002-1-RR

  HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IF Staining of HepG2 using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 80002-1-RR (TDP-43 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

• 

  FC experiment of HeLa using 80002-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 80002-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80002-1-RR against Human TDP-43 (N-terminal) were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, K-562-Zellen, Neuro-2a-Zellen
• Erfolgreiche IP: HAP1 cells, HeLa-Zellen
• Erfolgreiche Detektion in IHC-Autostainer: humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500
• Immunhistochemie (IHC): IHC : 1:1600-1:6400
• Immunfluoreszenz (IF)-P: IF-P : 1:800-1:3200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

80002-1-RR bindet in WB, IHC, IHC-Autostainer, IF/ICC, IF-P, FC (Intra), IP, ELISA TDP-43 (N-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TDP-43 (N-terminal) fusion protein Ag1231
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001487
• Gene symbol: TDP-43
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Patient-derived Induced Pluripotent Stem Cells as a Model to Study Frontotemporal Dementia Pathologies; Authors - Sonia Infante-Tadeo
human	IF	Nat Chem Biol; Small-molecule dissolution of stress granules by redox modulation benefits ALS models; Authors - Hiroyuki Uechi
mouse	WB,IF	Neural Regen Res; 5-Hydroxytryptamine: a potential therapeutic target in amyotrophic lateral sclerosis; Authors - Shi-Shi Jiang
human	WB,IP,IF	F1000Res; The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence; Authors - Donovan Worrall
human	IHC	J Neuropathol Exp Neurol; COVID-19 could worsen cerebral amyloid angiopathy; Authors - Julie Dewisme