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TDP-43 (C-terminal) Monoklonaler Antikörper

TDP-43 (C-terminal) Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G5F6

Kat-Nr. : 67345-1-PBS

Synonyme

TARDBP, TDP 43, TDP43, TDP-43, 1G5F6

Filter:
  • Western Blot (WB) analysis of HeLa cells using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    WB analysis of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

    WB result of TDP-43 (C-terminal) antibody (67345-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 (C-terminal) transfected HeLa cells. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    WB analysis using 67345-1-Ig (same clone as 67345-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    IHC staining of mouse brain using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    IHC staining of human gliomas using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    IHC staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    IF Staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 (C-terminal) Monoclonal antibody (67345-1-Ig)

    IF Staining of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (67345-1-Ig, Clone: 1G5F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67345-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA TDP-43 (C-terminal) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name TAR DNA binding protein
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 43-45 kDa
GenBank-ZugangsnummerNM_007375
Gene symbol TDP-43
Gene ID (NCBI) 23435
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.