TDP1 Polyklonaler Antikörper

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• KD/KO Validated

TDP1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10641-1-AP

Synonyme

Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, Tyr DNA phosphodiesterase 1, EC:3.1.4.-

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  WB analysis of MCF-7 using 10641-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10641-1-AP

  IP result of anti-TDP1 (IP:10641-1-AP, 4ug; Detection:10641-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human ovary cancer using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

10641-1-AP bindet in WB, IHC, IF, IP, ELISA TDP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TDP1 fusion protein Ag0964
• Vollständiger Name: tyrosyl-DNA phosphodiesterase 1
• Berechnetes Molekulargewicht: 34 kDa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC006083
• Gene symbol: TDP1
• Gene ID (NCBI): 55775
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).

Publikationen

Species	Application	Title
human	IF	Biochem Pharmacol; Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition.; Authors - Perego Paola P
human	WB	DNA Repair (Amst); TDP1 is required for efficient non-homologous end joining in human cells.; Authors - Jing Li ; KO Validated
human	WB	Int J Mol Sci; Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer; Authors - Inken Flörkemeier
human	WB	Anal Chem; Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling; Authors - Qiqi Wang