TDRKH Monoklonaler Antikörper

TDRKH Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B1B12

Kat-Nr. : 66845-1-PBS

Synonyme

TDRD2, TDRKH, tudor and KH domain containing

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  WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66845-1-Ig (same clone as 66845-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66845-1-Ig (same clone as 66845-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66845-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TDRKH und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TDRKH fusion protein Ag4720
• Vollständiger Name: tudor and KH domain containing
• Berechnetes Molekulargewicht: 606 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67-70 kDa
• GenBank-Zugangsnummer: BC032690
• Gene symbol: TDRKH
• Gene ID (NCBI): 11022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.