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TEAD3 Polyklonaler Antikörper

TEAD3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13120-1-PBS

Synonyme

DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3

Filter:
  • Western Blot (WB) analysis of various lysates using TEAD3 Polyclonal antibody (13120-1-AP)

    WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TEAD3 Polyclonal antibody (13120-1-AP)

    WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse intestine using TEAD3 Polyclonal antibody (13120-1-AP)

    IHC staining of mouse intestine using 13120-1-AP (same clone as 13120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TEAD3 Polyclonal antibody (13120-1-AP)

    IF Staining of MCF-7 using 13120-1-AP (same clone as 13120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

13120-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TEAD3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TEAD3 fusion protein Ag3875
Vollständiger Name TEA domain family member 3
Berechnetes Molekulargewicht 435 aa, 49 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC027877
Gene symbol TEAD3
Gene ID (NCBI) 7005
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.