TEAD4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TEAD4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(32)

Rezensionen (1)

Kat-Nr. : 12418-1-AP

Synonyme

EFTR 2, hRTEF 1B, RTEF1, TCF13L1, TEA domain family member 4

---

• 

  WB analysis using 12418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, HepG2-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 26 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Produktinformation

12418-1-AP bindet in WB, IF, IP, CoIP, ChIP, ELISA TEAD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEAD4 fusion protein Ag3059
• Vollständiger Name: TEA domain family member 4
• Berechnetes Molekulargewicht: 434 aa, 48 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC015497
• Gene symbol: TEAD4
• Gene ID (NCBI): 7004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 50-55 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang
human	WB	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen
mouse	WB	Free Radic Biol Med; Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury; Authors - Wei Deng
human	WB	Br J Pharmacol; An agonist of the adenosine A2A receptor, CGS21680, promotes corneal epithelial wound healing via the YAP signalling pathway; Authors - Qiuqin Sun
mouse,human	WB,IF	Clin Sci (Lond); IGFBP7 promotes endothelial cell repair in the recovery phase of acute lung injury; Authors - Rui He
human	CoIP	J Cell Mol Med; PMID: 31559693; Authors - Yangyang Yao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Pramod Bahadur (Verified Customer) (03-05-2024)

We used this antibody for PC3 cell lines with vector control, PDEF over expression and PDEF TWIST overexpression

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: PC3

TEAD4 Antibody Western Blot validation (1:2500 dilution) in PC3 (Cat no:12418-1-AP)