TEAD4 Rekombinanter Antikörper

TEAD4 Rekombinant Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242496B2

Kat-Nr. : 84932-4-PBS

Synonyme

242496B2, EFTR 2, hRTEF 1B, RTEF1, TCF13L1

---

• 

  WB analysis of MDA-MB-231 using 84932-4-RR (same clone as 84932-4-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

• 

  WB analysis of BGC-823 using 84932-4-RR (same clone as 84932-4-PBS)

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01682-3

  Sandwich ELISA standard curve of MP01682-3, Human TEAD4 Recombinant Matched Antibody Pair - PBS only. 84932-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3059. 84932-4-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

• 

  Sample test of MP01682-3

  The mean TEAD4 concentration was determined to be 8.65 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load and 6.31 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

• 

  IHC staining of human colon cancer using 84932-4-RR (same clone as 84932-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84932-4-RR (TEAD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84932-4-RR (same clone as 84932-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84932-4-RR (TEAD4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84932-4-RR (same clone as 84932-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TEAD4 antibody (84932-4-RR, Clone: 242496B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84932-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84932-4-RR against Human TEAD4 were performed. The affinity constant is 0.205 nM.

---

Geprüfte Anwendungen

Produktinformation

84932-4-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test TEAD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TEAD4 fusion protein Ag3059
• Vollständiger Name: TEA domain family member 4
• Berechnetes Molekulargewicht: 434 aa, 48 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC015497
• Gene symbol: TEAD4
• Gene ID (NCBI): 7004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEAD4 belongs to a protein family that shares the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins, a TBP-associated factor, and a limiting transcriptional intermediary factor. TEAD4 also performs an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration, and epithelial-mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but the molecular weight of TEAD4 is detected at about 50-55 kDa.