TEKT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TEKT1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 18968-1-AP

Synonyme

Tektin-1

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  WB analysis of human brain using 18968-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 18968-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 18968-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18968-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18968-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 18968-1-AP

  Immunofluorescent analysis of HepG2 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 18968-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Hodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

18968-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TEKT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEKT1 fusion protein Ag5173
• Vollständiger Name: tektin 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC014599
• Gene symbol: TEKT1
• Gene ID (NCBI): 83659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB,IP	Cell Mol Life Sci; Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella; Authors - Xin-Yan Geng
human	WB,IF	Hum Mol Genet; Functional characterization of tektin-1 in motile cilia and evidence for TEKT1 as a new candidate gene for motile ciliopathies.; Authors - Rebecca Ryan ; KD Validated
mouse	IF	Dev Dyn; Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects; Authors - Pei-Yi Pan