TELO2 Monoklonaler Antikörper

TELO2 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H10F4

Kat-Nr. : 66077-1-PBS

Synonyme

TEL2, Protein clk-2 homolog, Protein clk 2 homolog, KIAA0683, hCLK2

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  WB analysis using 66077-1-Ig (same clone as 66077-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66077-1-Ig (same clone as 66077-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66077-1-Ig (same clone as 66077-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66077-1-Ig (same clone as 66077-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50016-1

  Sandwich ELISA standard curve of MP50016-1, TELO2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66077-2-PBS. Detection antibody: HRP-conjugated 66077-1-PBS. Standard: Ag8834. Range: 0.156-20 ng/mL.

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  IP experiment of HepG2 using 66077-1-Ig (same clone as 66077-1-PBS)

  IP result of anti-TELO2 (IP:66077-1-Ig, 4ug; Detection:66077-1-Ig 1:500) with HepG2 cells lysate 2900ug. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66077-1-Ig (same clone as 66077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66077-1-Ig (same clone as 66077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66077-1-Ig (same clone as 66077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66077-1-Ig (same clone as 66077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66077-1-Ig (same clone as 66077-1-PBS)

  Immunofluorescent analysis of () fixed HepG2 cells using 66077-1-Ig (TELO2 antibody) at dilution of 1:25. This data was developed using the same antibody clone with 66077-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50016-1

  Cytometric bead array standard curve of MP50016-1, TELO2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66077-2-PBS. Detection antibody: 66077-1-PBS. Standard: Ag8834. Range: 1.563-12.5 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66077-1-PBS bindet in WB, IHC, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA TELO2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TELO2 fusion protein Ag8834
• Vollständiger Name: TEL2, telomere maintenance 2, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 837 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC017188
• Gene symbol: TELO2
• Gene ID (NCBI): 9894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), and ultraviolet (UV). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.