TEM1 Monoklonaler Antikörper

TEM1 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9B4

Kat-Nr. : 60170-1-PBS

Synonyme

CD248, 1F9B4, CD164L1, CD248 molecule, endosialin, Endosialin

---

• 

  WB analysis using 60170-1-Ig (same clone as 60170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody) at dilution of 1:23000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 60170-1-Ig (same clone as 60170-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 60170-1-Ig (same clone as 60170-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:1800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51052-1

  Cytometric bead array standard curve of MP51052-1, CD248 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60170-1-PBS. Detection antibody: 60170-3-PBS. Standard:Ag13334. Range: 6.25-200 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

60170-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA TEM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TEM1 fusion protein Ag13334
• Vollständiger Name: CD248 molecule, endosialin
• Berechnetes Molekulargewicht: 757 aa, 81 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC051340
• Gene symbol: TEM1
• Gene ID (NCBI): 57124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.