TERF2 Monoklonaler Antikörper

TERF2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

5B1E1

Rezensionen (1)

Kat-Nr. : 66893-1-Ig

Synonyme

5B1E1, Telomeric DNA-binding protein, Telomeric repeat-binding factor 2, TRBF2, TRF2

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  WB analysis of MCF-7 using 66893-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66893-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66893-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66893-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66893-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66893-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66893-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66893-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66893-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66893-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66893-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, 4T1-Zellen, Daudi-Zellen, HEK-293-Zellen, HT-29-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

66893-1-Ig bindet in WB, ChIP, ELISA TERF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TERF2 fusion protein Ag28513
• Vollständiger Name: telomeric repeat binding factor 2
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 60-65.32-35 kDa
• GenBank-Zugangsnummer: BC024890
• Gene symbol: TERF2
• Gene ID (NCBI): 7014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.

Publikationen

Species	Application	Title
human		Sci Adv; Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).; Authors - Ji Hoon Lee
mouse,human	WB	Front Pharmacol; Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.; Authors - Xiaoyu Qi
human	WB,ChIP	Nat Commun; Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence; Authors - Athanasios Siametis
mouse	WB	Acta Biochim Biophys Sin (Shanghai); miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2; Authors - Yaqing Jiang
mouse	WB	Exp Mol Med; SUMOylation of TP53INP1 is involved in miR-30a-5p-regulated heart senescence; Authors - Yi-Xiang Hong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Refik (Verified Customer) (08-26-2025)

Antibody works well when prepared at 1:5000 dilution in 5% BSA and overnight primary incubation for Western Blot with 1:300 secondary antibody and 1:500 dilution using 5% Donkey Serum for ICC/IHC for overnight incubation with 1:400 secondary (2 hours at rtp) and 1:10000 DAPI for nuclei.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:5000 for Western Blot and 1:500 for ICC/IHC for both human-derived cells
• Cell Tissue Type: Human cardiac cells