TERF2IP Polyklonaler Antikörper

TERF2IP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14595-1-PBS

Synonyme

Telomeric repeat-binding factor 2-interacting protein 1, RAP1 homolog, PP8000, hRap1, DRIP5

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  WB analysis of HEK-293 using 14595-1-AP (same clone as 14595-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14595-1-AP (same clone as 14595-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 14595-1-AP (same clone as 14595-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 14595-1-AP (same clone as 14595-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 14595-1-AP (same clone as 14595-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 14595-1-AP (same clone as 14595-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  IP result of anti-TERF2IP (IP:14595-1-AP, 3ug; Detection:14595-1-AP 1:800) with HeLa cells lysate 2720ug. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 14595-1-AP (same clone as 14595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TERF2IP antibody (14595-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14595-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TERF2IP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TERF2IP fusion protein Ag6130
• Vollständiger Name: telomeric repeat binding factor 2, interacting protein
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC005841
• Gene symbol: TERF2IP
• Gene ID (NCBI): 54386
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)