TERT Polyklonaler Antikörper

TERT Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 27586-1-AP

Synonyme

EC:2.7.7.49, EST2, hEST2, hTERT, TCS1

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  WB analysis using 27586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27586-1-AP (TERT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Rattenlungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

27586-1-AP bindet in WB, IF, ELISA TERT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TERT fusion protein Ag26270
• Vollständiger Name: telomerase reverse transcriptase
• Berechnetes Molekulargewicht: 127 kDa
• Beobachtetes Molekulargewicht: ~100 kDa
• GenBank-Zugangsnummer: NM_001193376
• Gene symbol: TERT
• Gene ID (NCBI): 7015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase. TERT plays a key role in cancer formation, ensuring chromosomal stability by maintaining telomere length, and allowing cells to avert senescence. It constitutes a limiting factor for formation of the telomerase complex in cancer cells. TERT is one of two major components of the larger telomerase complex, which extends telomeres by adding specific short repetitive DNA sequences. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. The calculated MW of TERT is 127 kDa, 27586-1-AP can detect a band around 100 kDa.

Publikationen

Species	Application	Title
mouse,human	WB,IF	Chem Biol Interact; Telomerase reverse transcriptase protects against diabetic kidney disease by promoting AMPK/PGC-1a-regulated mitochondrial energy homeostasis; Authors - Nan Ma
human	WB	J Tissue Eng; Establishing an hTERT-driven immortalized umbilical cord-derived mesenchymal stem cell line and its therapeutic application in mice with liver failure; Authors - Qi Chen
mouse	WB	J Inflamm Res; FTO Stabilizes MIS12 to Inhibit Vascular Smooth Muscle Cell Senescence in Atherosclerotic Plaque; Authors - Jingzhao Sun
human	WB	Transl Cancer Res; Effect and mechanism of curcumin on colon cancer cell senescence through early growth response 1 (EGR1); Authors - Dan Xu