TESC Polyklonaler Antikörper

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• KD/KO Validated

TESC Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 11125-1-AP

Synonyme

CHP3, TESC, tescalcin, TSC

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Galerie der Validierungsdaten

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  WB analysis using 11125-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11125-1-AP (TESC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11125-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11125-1-AP (TESC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 11125-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11125-1-AP (TESC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11125-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11125-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11125-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11125-1-AP (TESC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 11125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 11125-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11125-1-AP (TESC antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreaskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 7 publications below

Produktinformation

11125-1-AP bindet in WB, IHC, IF, ELISA TESC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TESC fusion protein Ag1596
• Vollständiger Name: tescalcin
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC015221
• Gene symbol: TESC
• Gene ID (NCBI): 54997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Genome Biol; Long non-coding RNA ROR decoys gene-specific histone methylation to promote tumorigenesis.; Authors - Jiayan Fan
mouse	WB	J Neurosci; CHP1-Mediated NHE1 Biosynthetic Maturation Is Required for Purkinje Cell Axon Homeostasis.; Authors - Liu Ye Y
human	WB,IHC	Cancer Commun (Lond); Tescalcin is an unfavorable prognosis factor that regulats cell proliferation and survival in hepatocellular carcinoma patients.; Authors - Zhong-Guo Zhou ; KD Validated
human	IHC	Front Oncol; The Expression Profile, Clinical Application and Potential Tumor Suppressing Mechanism of hsa_circ_0001675 in Head and Neck Carcinoma.; Authors - Yujie Cao
human	WB	Oncol Rep; Differential expression of tescalcin by modification of promoter methylation controls cell survival in gastric cancer cells.; Authors - Tae Woo Kim
human	IHC	BMC Cancer; Tescalcin promotes highly invasive papillary thyroid microcarcinoma by regulating FOS/ERK signaling pathway.; Authors - Xiuhe Zou