TEX14 Polyklonaler Antikörper

TEX14 Polyklonal Antikörper für IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18351-1-PBS

Synonyme

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  WB analysis of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18351-1-AP (TEX14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18351-1-PBS bindet in IHC, Indirect ELISA TEX14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEX14 fusion protein Ag13177
• Vollständiger Name: testis expressed 14
• Berechnetes Molekulargewicht: 957aa,107 kDa; 1497aa,168 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa, 106 kDa
• GenBank-Zugangsnummer: BC040526
• Gene symbol: TEX14
• Gene ID (NCBI): 56155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.