TEX14 Monoklonaler Antikörper

TEX14 Monoklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11D6

Kat-Nr. : 67982-1-PBS

Synonyme

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

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  WB analysis of rat testis using 67982-1-Ig (same clone as 67982-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit testis using 67982-1-Ig (same clone as 67982-1-PBS)

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67982-1-Ig (same clone as 67982-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67982-1-PBS bindet in WB, IF-P, Indirect ELISA TEX14 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TEX14 fusion protein Ag13143
• Vollständiger Name: testis expressed 14
• Berechnetes Molekulargewicht: 957aa,107 kDa; 1497aa,168 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC040526
• Gene symbol: TEX14
• Gene ID (NCBI): 56155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.