TEX264 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TEX264 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 25858-1-AP

Synonyme

SIG11, TEG 264, Testis-expressed protein 264, TEX 264, UNQ337/PRO536

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  WB analysis using 25858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25858-1-AP

  WB result of TEX264 antibody (25858-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX264 transfected HEK-293 cells.

• 

  WB analysis using 25858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 25858-1-AP

  IP result of anti-TEX264 (IP:25858-1-AP, 4ug; Detection:25858-1-AP 1:3000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of mouse testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 25858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TEX264 antibody (25858-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Neuro-2a-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 4 publications below

Produktinformation

25858-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TEX264 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEX264 fusion protein Ag23027
• Vollständiger Name: testis expressed 264
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC008742
• Gene symbol: TEX264
• Gene ID (NCBI): 51368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

Publikationen

Species	Application	Title
human	WB	Nat Commun; AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity; Authors - Linliang Zhang
human	IF	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
mouse	WB,IF	ACS Chem Neurosci; Anti-Inflammatory Anthranilate Analogue Enhances Autophagy through mTOR and Promotes ER-Turnover through TEX264 during Alzheimer-Associated Neuroinflammation.; Authors - Zhiyu Wang ; KD Validated
mouse	WB	Biomedicines; Enhancement of TEX264-Mediated ER-Phagy Contributes to the Therapeutic Effect of Glycycoumarin against APA Hepatotoxicity in Mice.; Authors - Mingzhu Yan
human	WB,IF	Mol Biol Cell; Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin.; Authors - Andrew Kennedy
human	WB	Mol Biol Cell; CCPG1 recognizes ER luminal proteins for selective ER-phagy; Authors - Shunsuke Ishii