TEX264 Rekombinanter Antikörper

TEX264 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242500B3

Kat-Nr. : 84946-5-RR

Synonyme

242500B3, SIG11, TEG 264, Testis-expressed protein 264, TEX 264

---

• 

  WB analysis using 84946-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84946-5-RR (TEX264 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 84946-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84946-5-RR (TEX264 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 84946-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TEX264 antibody (84946-5-RR, Clone: 242500B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84946-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84946-5-RR against Human TEX264 were performed. The affinity constant is 0.254 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84946-5-RR bindet in WB, IHC, IF/ICC, ELISA TEX264 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TEX264 fusion protein Ag23027
• Vollständiger Name: testis expressed 264
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC008742
• Gene symbol: TEX264
• Gene ID (NCBI): 51368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.