Transferrin/TF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Transferrin/TF Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(88)

Rezensionen (1)

Kat-Nr. : 17435-1-AP

Synonyme

TF, transferrin, Beta 1 metal binding globulin, Beta-1 metal-binding globulin, PRO1557

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  WB analysis using 17435-1-AP

  human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17435-1-AP

  WB result of Transferrin/TF antibody (17435-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Transferrin/TF transfected HepG2 cells.

• 

  WB analysis of mouse kidney using 17435-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17435-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17435-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17435-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17435-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17435-1-AP (Transferrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human placenta using 17435-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Transferrin/TF antibody (17435-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17435-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humane Urinprobe, HepG2-Zellen, Mausnierengewebe, Mausmilzgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 74 publications below
• IHC: See 12 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

17435-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Transferrin/TF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Ente, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Transferrin/TF fusion protein Ag11504
• Vollständiger Name: transferrin
• Berechnetes Molekulargewicht: 698 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC059367
• Gene symbol: TF
• Gene ID (NCBI): 7018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

Publikationen

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	IF	Nat Neurosci; Myelin-axon interface vulnerability in Alzheimer's disease revealed by subcellular proteomics and imaging of human and mouse brain; Authors - Yifei Cai
	WB	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse,human	WB	Sci Adv; The adipocyte-enriched secretory protein tetranectin exacerbates type 2 diabetes by inhibiting insulin secretion from β cells; Authors - Fen Liu
human	WB	Dev Cell; Lactylation of LSD1 is an acquired epigenetic vulnerability of BRAFi/MEKi-resistant melanoma; Authors - Aicun Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (11-01-2024)

great with icc

• Applications: Immunohistochemistry