TFAM Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TFAM Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

108

Rezensionen (2)

Kat-Nr. : 22586-1-AP

Synonyme

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Galerie der Validierungsdaten

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  WB analysis using 22586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22586-1-AP

  IP Result of anti-TFAM (IP:22586-1-AP, 4ug; Detection:22586-1-AP 1:800) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 99 publications below
• IHC: See 10 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

22586-1-AP bindet in WB, IP, IHC, IF, chIP, ELISA TFAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFAM fusion protein Ag18299
• Vollständiger Name: transcription factor A, mitochondrial
• Berechnetes Molekulargewicht: 246 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publikationen

Species	Application	Title
	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
	WB，IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
	IHC	Cell Metab; Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.; Authors - Ki Wung Chung
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sebastian (Verified Customer) (10-25-2023)

Good detection signals for TFAM in western blot of liver from old and young mice, some unspecific bands were slightly visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: liver

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain