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TFAM Polyklonaler Antikörper

TFAM Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(178)

Kat-Nr. : 22586-1-AP

Synonyme

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22586-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA TFAM und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Huhn, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TFAM fusion protein Ag18299
Vollständiger Name transcription factor A, mitochondrial
Berechnetes Molekulargewicht 246 aa, 29 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC126366
Gene symbol TFAM
Gene ID (NCBI) 7019
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TFAM antibody 22586-1-APProtokoll herunterladen
IHC protocol for TFAM antibody 22586-1-APProtokoll herunterladenl
IF protocol for TFAM antibody 22586-1-APProtokoll herunterladen
IP protocol for TFAM antibody 22586-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
IHC

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
WB,IHC

Circulation

Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.

Authors - Jorge Oller
IHC

Cell Metab

Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.

Authors - Ki Wung Chung
humanWB

Nat Cell Biol

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Authors - Timo Rey

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Sneha (Verified Customer) (09-24-2025)

GOOD

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ARPE-19
FH

Emilie (Verified Customer) (09-24-2025)

Produced accurate detection at the expected molecular size range.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fibroblasts
FH

Michael (Verified Customer) (09-23-2025)

Works great!!

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Corneal cells
FH

Sebastian (Verified Customer) (10-25-2023)

Good detection signals for TFAM in western blot of liver from old and young mice, some unspecific bands were slightly visible

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: liver
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain