TFAM Monoklonaler Antikörper

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• KD/KO Validated

TFAM Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8H3

Kat-Nr. : 68557-5-Ig

Synonyme

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

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  WB analysis using 68557-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68557-5-Ig

  WB result of TFAM antibody (68557-5-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAM transfected HEK-293 cells.

• 

  WB analysis using 68557-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 68557-5-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 68557-5-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68557-5-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (68557-5-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Caco-2-Zellen, HEK-293-Zellen, K-562-Zellen, LNCaP-Zellen, MOLT-4-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68557-5-Ig bindet in WB, IHC, IF/ICC, ELISA TFAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TFAM fusion protein Ag18253
• Vollständiger Name: transcription factor A, mitochondrial
• Berechnetes Molekulargewicht: 246 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.