TFAM Monoklonaler Antikörper

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TFAM Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8H3

Kat-Nr. : 68557-5-PBS

Synonyme

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

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  WB analysis using 68557-5-Ig (same clone as 68557-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68557-5-Ig (same clone as 68557-5-PBS)

  WB result of TFAM antibody (68557-5-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAM transfected HEK-293 cells. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  WB analysis using 68557-5-Ig (same clone as 68557-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (68557-5-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68557-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TFAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TFAM fusion protein Ag18253
• Vollständiger Name: transcription factor A, mitochondrial
• Berechnetes Molekulargewicht: 246 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.