TFAM Rekombinanter Antikörper

TFAM Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4L4

Kat-Nr. : 82745-1-PBS

Synonyme

Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1

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  WB analysis using 82745-1-RR (same clone as 82745-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82745-1-RR (same clone as 82745-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82745-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82745-1-RR against Human TFAM were performed. The affinity constant is 45.7 pM.

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Geprüfte Anwendungen

Produktinformation

82745-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TFAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TFAM fusion protein Ag18299
• Vollständiger Name: transcription factor A, mitochondrial
• Berechnetes Molekulargewicht: 246 aa, 29 kDa
• Beobachtetes Molekulargewicht: 24-25 kDa
• GenBank-Zugangsnummer: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.