TFAP2A,AP-2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TFAP2A,AP-2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 13019-3-AP

Synonyme

Activator protein 2, AP 2, AP 2 transcription factor, AP 2alpha, AP2, AP2 alpha, AP2TF, BOFS, TFAP2, TFAP2A, TFAP2A,AP-2

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Galerie der Validierungsdaten

• 

  WB analysis using 13019-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13019-3-AP (TFAP2A,AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 13019-3-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13019-3-AP (TFAP2A,AP-2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13019-3-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAP2A,AP-2 antibody (13019-3-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

13019-3-AP bindet in WB, IF, ChIP, ELISA TFAP2A,AP-2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFAP2A,AP-2 fusion protein Ag4112
• Vollständiger Name: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• Berechnetes Molekulargewicht: 431 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC017754
• Gene symbol: TFAP2A
• Gene ID (NCBI): 7020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression (PMID: 21966377). TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation (PMID: 11694877).

Publikationen

Species	Application	Title
		Oncol Rep; Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma.; Authors - Ming-Xu Qiao
human, mouse	WB, ChIP	Acta Pharmacol Sin; Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.; Authors - Zhan-Ping Lu ; KD Validated
	ChIP	Cell Death Dis; TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-β signaling pathway.; Authors - Yanlu Xiong
human	WB	Cell Death Dis; The m6A modification-mediated OGDHL exerts a tumor suppressor role in ccRCC by downregulating FASN to inhibit lipid synthesis and ERK signaling; Authors - Jian Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

• Applications: Immunofluorescence
• Cell Tissue Type: Retinal organoids