TFAP2A/AP-2 Monoklonaler Antikörper

TFAP2A/AP-2 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1B12F11

Kat-Nr. : 67076-1-PBS

Synonyme

TFAP2A, TFAP2A,AP-2, 1B12F11, Activating enhancer-binding protein 2-alpha, AP 2

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  WB analysis of L02 using 67076-1-Ig (same clone as 67076-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67076-1-Ig (same clone as 67076-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67076-1-Ig (same clone as 67076-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67076-1-Ig (same clone as 67076-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67076-1-Ig (same clone as 67076-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67076-1-Ig (same clone as 67076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67076-1-Ig (same clone as 67076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 67076-1-Ig (same clone as 67076-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TFAP2A/AP-2 (67076-1-Ig, Clone:1B12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67076-1-PBS bindet in WB, IHC, FC (Intra), ELISA TFAP2A/AP-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TFAP2A/AP-2 fusion protein Ag4112
• Vollständiger Name: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• Berechnetes Molekulargewicht: 431 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC017754
• Gene symbol: TFAP2A/AP-2
• Gene ID (NCBI): 7020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression. [PMID:21966377] TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation [PMID:11694877]