TFAP2E Polyklonaler Antikörper

TFAP2E Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25829-1-AP

Synonyme

AP2 epsilon, AP2E, TFAP2E

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  WB analysis of A375 using 25829-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25829-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 25829-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 25829-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25829-1-AP (TFAP2E Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 25829-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25829-1-AP (TFAP2E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 25829-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25829-1-AP (TFAP2E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 25829-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 25829-1-AP (TFAP2E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

25829-1-AP bindet in WB, IHC, IF/ICC, ELISA TFAP2E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFAP2E fusion protein Ag23023
• Vollständiger Name: transcription factor AP-2 epsilon (activating enhancer binding protein 2 epsilon)
• Berechnetes Molekulargewicht: 442 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC041175
• Gene symbol: TFAP2E
• Gene ID (NCBI): 339488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Elife; Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons; Authors - Jennifer M Lin