TFAP4 Polyklonaler Antikörper

TFAP4 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12017-1-AP

Synonyme

AP 4, bHLHc41, TFAP4, Transcription factor AP 4

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  WB analysis using 12017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12017-1-AP

  IP result of anti-TFAP4 (IP:12017-1-AP, 4ug; Detection:12017-1-AP 1:1000) with HeLa cells lysate 1360 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, NCI-H1299-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12017-1-AP bindet in WB, IHC, IP, ChIP, ELISA TFAP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFAP4 fusion protein Ag2633
• Vollständiger Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Berechnetes Molekulargewicht: 338 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC010576
• Gene symbol: TFAP4
• Gene ID (NCBI): 7023
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,ChIP	Front Immunol; Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease; Authors - Enyi Zhu
human	WB	J Agric Food Chem; Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling.; Authors - Peng Yang
human	WB	J Agric Food Chem; Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway.; Authors - Peng Yang
human	WB	Invest Ophthalmol Vis Sci; A Novel Small Peptide H-KI20 Inhibits Retinal Neovascularization Through the JNK/ATF2 Signaling Pathway.; Authors - Ruonan Wang
human	WB	Sci Adv; Genome-wide CRISPR screen identifies IRF1 and TFAP4 as transcriptional regulators of Galectin-9 in T cell acute lymphoblastic leukemia; Authors - Caroline R M Wiggers ; KO Validated