TFAP4 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TFAP4 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240031D5

Kat-Nr. : 83196-4-RR

Synonyme

240031D5, Activating enhancer-binding protein 4, AP 4, bHLHc41, Class C basic helix-loop-helix protein 41

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  WB analysis using 83196-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83196-4-RR (TFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 83196-4-RR

  WB result of TFAP4 antibody (83196-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAP4 transfected HeLa cells.

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  IF Staining of HeLa using 83196-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFAP4 antibody (83196-4-RR, Clone: 240031D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83196-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83196-4-RR against Human TFAP4 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83196-4-RR bindet in WB, IF/ICC, ELISA TFAP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TFAP4 fusion protein Ag2633
• Vollständiger Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Berechnetes Molekulargewicht: 338 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC010576
• Gene symbol: TFAP4
• Gene ID (NCBI): 7023
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.