TFEB Rekombinanter Antikörper

TFEB Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242093D11

Kat-Nr. : 84675-5-RR

Synonyme

242093D11, Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35

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  WB analysis using 84675-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84675-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (84675-5-RR, Clone: 242093D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84675-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84675-5-RR bindet in WB, IF/ICC, ELISA TFEB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TFEB fusion protein Ag4194
• Vollständiger Name: transcription factor EB
• Berechnetes Molekulargewicht: 476 aa, 53 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC032448
• Gene symbol: TFEB
• Gene ID (NCBI): 7942
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).