TFF1 Polyklonaler Antikörper

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• KD/KO Validated

TFF1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13734-1-PBS

Synonyme

Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A

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  WB analysis using 13734-1-AP (same clone as 13734-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13734-1-AP (TFF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 13734-1-AP (same clone as 13734-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (TFF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 13734-1-AP (same clone as 13734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13734-1-AP (same clone as 13734-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13734-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TFF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFF1 fusion protein Ag4541
• Vollständiger Name: trefoil factor 1
• Berechnetes Molekulargewicht: 84 aa, 9 kDa
• Beobachtetes Molekulargewicht: 9-12 kDa
• GenBank-Zugangsnummer: BC032811
• Gene symbol: TFF1
• Gene ID (NCBI): 7031
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.