TFF1 Rekombinanter Antikörper

TFF1 Rekombinant Antikörper für FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240424F5

Kat-Nr. : 83467-2-PBS

Synonyme

240424F5, Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2

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  FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83467-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83467-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

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Geprüfte Anwendungen

Produktinformation

83467-2-PBS bindet in FC (Intra), Cytometric bead array, Indirect ELISA TFF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TFF1 fusion protein Ag4541
• Vollständiger Name: trefoil factor 1
• Berechnetes Molekulargewicht: 84 aa, 9 kDa
• GenBank-Zugangsnummer: BC032811
• Gene symbol: TFF1
• Gene ID (NCBI): 7031
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.