TFF2 Polyklonaler Antikörper

TFF2 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13681-1-PBS

Synonyme

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  WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13681-1-AP (same clone as 13681-1-PBS)

  Human urine tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  human stomach sample were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  IP result of anti-TFF2 (IP:13681-1-AP, 4ug; Detection:13681-1-AP 1:600) with mouse stomach tissue lysate 4000ug. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13681-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA TFF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFF2 fusion protein Ag4537
• Vollständiger Name: trefoil factor 2
• Berechnetes Molekulargewicht: 129 aa, 14 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC032820
• Gene symbol: TFF2
• Gene ID (NCBI): 7032
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).