TFF2 Rekombinanter Antikörper

TFF2 Rekombinant Antikörper für WB, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240661G12

Kat-Nr. : 83717-4-PBS

Synonyme

SML1, 240661G12, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin

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  WB analysis of human urine using 83717-4-RR (same clone as 83717-4-PBS)

  Human urine sample was subjected to SDS PAGE followed by western blot with 83717-4-RR (TFF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83717-4-PBS in a different storage buffer formulation.

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  IP experiment of mouse stomach using 83717-4-RR (same clone as 83717-4-PBS)

  IP result of anti-TFF2 (IP:83717-4-RR, 4ug; Detection:83717-4-RR 1:1000) with mouse stomach tissue lysate 1360 ug. This data was developed using the same antibody clone with 83717-4-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 83717-4-RR (same clone as 83717-4-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug TFF2 Recombinant antibody (83717-4-RR, Clone:240661G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83717-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83717-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83717-4-RR against Human TFF2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83717-4-PBS bindet in WB, FC (Intra), IP, ELISA TFF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TFF2 fusion protein Ag4537
• Vollständiger Name: trefoil factor 2
• Berechnetes Molekulargewicht: 129 aa, 14 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC032820
• Gene symbol: TFF2
• Gene ID (NCBI): 7032
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).