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TFIIE Beta Polyklonaler Antikörper

TFIIE Beta Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11596-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of K-562 cells using TFIIE Beta Polyclonal antibody (11596-1-AP)

    WB analysis of K-562 using 11596-1-AP (same clone as 11596-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using TFIIE Beta Polyclonal antibody (11596-1-AP)

    WB analysis of A375 using 11596-1-AP (same clone as 11596-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TFIIE Beta Polyclonal antibody (11596-1-AP)

    WB analysis of Jurkat using 11596-1-AP (same clone as 11596-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using TFIIE Beta Polyclonal antibody (11596-1-AP)

    IF Staining of A375 using 11596-1-AP (same clone as 11596-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using TFIIE Beta antibody (11596-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11596-1-PBS bindet in WB, IF/ICC, Indirect ELISA TFIIE Beta und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TFIIE Beta fusion protein Ag2136
Vollständiger Name general transcription factor IIE, polypeptide 2, beta 34kDa
Berechnetes Molekulargewicht 291 aa, 33 kDa
Beobachtetes Molekulargewicht 34 kDa
GenBank-ZugangsnummerBC030572
Gene symbol GTF2E2
Gene ID (NCBI) 2961
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).