TFIP11 Monoklonaler Antikörper

TFIP11 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1C8

Kat-Nr. : 66114-1-PBS

Synonyme

1A1C8, bK445C9.6, hNtr1, HSPC006, NTR1

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  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66114-1-Ig (same clone as 66114-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66114-1-Ig (same clone as 66114-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66114-1-PBS bindet in WB, IHC, IF/ICC, ELISA TFIP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TFIP11 fusion protein Ag5821
• Vollständiger Name: tuftelin interacting protein 11
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC033080
• Gene symbol: TFIP11
• Gene ID (NCBI): 24144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.