TFPI Polyklonaler Antikörper

TFPI Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20865-1-AP

Synonyme

EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20865-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20865-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20865-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20865-1-AP (TFPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

20865-1-AP bindet in WB, IHC, IF, ELISA TFPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFPI fusion protein Ag14913
• Vollständiger Name: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Berechnetes Molekulargewicht: 251aa,29 kDa; 304aa,35 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC015514
• Gene symbol: TFPI
• Gene ID (NCBI): 7035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Publikationen

Species	Application	Title
human	WB	Gene; ADTRP regulates TFPI expression via transcription factor POU1F1 involved in coronary artery disease.; Authors - Chunyan Luo
human	IHC	Clin Appl Thromb Hemost; Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.; Authors - Kang An
human	WB	Thromb Res; Activated protein C, protein S, and tissue factor pathway inhibitor cooperate to inhibit thrombin activation; Authors - Xian Li