TFPI Monoklonaler Antikörper

TFPI Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B12F11

Kat-Nr. : 66842-1-PBS

Synonyme

1B12F11, EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor

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  WB analysis of human placenta using 66842-1-Ig (same clone as 66842-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66842-1-Ig (same clone as 66842-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66842-1-Ig (same clone as 66842-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TFPI antibody (66842-1-Ig, Clone: 1B12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66842-1-Ig (same clone as 66842-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (66842-1-Ig, Clone: 1B12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66842-1-PBS bindet in WB, IF/ICC, Indirect ELISA TFPI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TFPI fusion protein Ag14980
• Vollständiger Name: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Berechnetes Molekulargewicht: 251aa,29 kDa; 304aa,35 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC015514
• Gene symbol: TFPI
• Gene ID (NCBI): 7035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.