TFPT Polyklonaler Antikörper

TFPT Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 10097-2-AP

Synonyme

TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1

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  WB analysis of K-562 using 10097-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10097-2-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10097-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10097-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10097-2-AP (TFPT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10097-2-AP bindet in WB, IHC, ELISA TFPT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFPT fusion protein Ag0131
• Vollständiger Name: TCF3 (E2A) fusion partner (in childhood Leukemia)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC001728
• Gene symbol: TFPT
• Gene ID (NCBI): 29844
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.

Publikationen

Species	Application	Title
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
mouse	WB	EMBO J; Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.; Authors - Christian Schutt

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