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- KD/KO Validated
CD71 Polyklonaler Antikörper
CD71 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (4)
Anwendung
WB, IP, IHC, IF, Cell treatment, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10084-2-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HT-1080.Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, K-562-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | humanes Plazenta-Gewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| Immunfluoreszenz (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 65 publications below |
| IHC | See 18 publications below |
| IF | See 4 publications below |
Produktinformation
10084-2-AP bindet in WB, IP, IHC, IF, Cell treatment, ELISA CD71 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Affe, Ente, Hausschwein, Huhn |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | CD71 fusion protein Ag0123 |
| Vollständiger Name | transferrin receptor (p90, CD71) |
| Berechnetes Molekulargewicht | 85 kDa |
| Beobachtetes Molekulargewicht | 90 kDa |
| GenBank-Zugangsnummer | BC001188 |
| Gene symbol | TFRC |
| Gene ID (NCBI) | 7037 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for CD71 antibody 10084-2-AP | Protokoll herunterladen |
| IHC protocol for CD71 antibody 10084-2-AP | Protokoll herunterladen |
| IF protocol for CD71 antibody 10084-2-AP | Protokoll herunterladen |
| IP protocol for CD71 antibody 10084-2-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Acta Neuropathol Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy. | ||
Cell Death Differ Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis | ||
Acta Biomater Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes | ||
Cancer Res Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nu (Verified Customer) (10-24-2023) | Although there is a band close to 100 kd, there are other even more stronger non-specific bands.
 |
FH Sara (Verified Customer) (10-25-2020) | Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.
 |
