Thyroglobulin Polyklonaler Antikörper

Thyroglobulin Polyklonal Antikörper für IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21714-1-AP

Synonyme

AITD3, TG, TGN, thyroglobulin

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Galerie der Validierungsdaten

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  IHC staining of human thyroid using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21714-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21714-1-AP (Thyroglobulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Schilddrüsengewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

21714-1-AP bindet in WB, IHC, IF, CoIP, ELISA Thyroglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Thyroglobulin fusion protein Ag16343
• Vollständiger Name: thyroglobulin
• Berechnetes Molekulargewicht: 2768 aa, 305 kDa
• GenBank-Zugangsnummer: BC140933
• Gene symbol: TG
• Gene ID (NCBI): 7038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	Oncotarget; HER inhibitor promotes BRAF/MEK inhibitor-induced redifferentiation in papillary thyroid cancer harboring BRAFV600E.; Authors - Lingxiao Cheng
	CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.; Authors - Madison T Wright
human	IF	J Lipid Res; Differential contributions of choline phosphotransferases CPT1 and CEPT1 to the biosynthesis of choline phospholipid.; Authors - Yasuhiro Horibata
human	WB	Cancer Lett; Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer; Authors - Zongfu Pan