TGF Beta 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TGF Beta 1 Polyklonal Antikörper für WB, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(770)

Rezensionen (10)

Kat-Nr. : 21898-1-AP

Synonyme

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB

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  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21898-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21898-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human stomach cancer using 21898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 21898-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, A549-Zellen, HeLa-Zellen, K-562-Zellen, L-929-Zellen, Mausmilzgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IF-P: humanes Magenkrebsgewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 606 publications below
• IHC: See 171 publications below
• IF: See 82 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

21898-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA TGF Beta 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Hund, Kaninchen, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGF Beta 1 fusion protein Ag13591
• Vollständiger Name: transforming growth factor, beta 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000125
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.

Publikationen

Species	Application	Title
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
	WB,IF	Acta Pharm Sin B; Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment.; Authors - Rong Guo
mouse	IF	Adv Sci (Weinh); M2 Macrophage-Derived Extracellular Vesicles Reprogram Immature Neutrophils into Anxa1hi Neutrophils to Enhance Inflamed Bone Regeneration; Authors - Yufei Yao
human	WB	Dev Cell; Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation; Authors - Bin Tan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Javier (Verified Customer) (09-09-2025)

Good antibody for WB assay.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice kidneys

FH

Juhi (Verified Customer) (07-23-2025)

Antibody works fine, giving correct band size in western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Aorta

FH

Andrea (Verified Customer) (03-22-2024)

We had a good confocal signal and beautiful bands in our experimental group of high TGFb

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:50-1:500
• Cell Tissue Type: guinea pig myocardium

FH

Kenzo (Verified Customer) (08-15-2023)

This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse Kidney

FH

Celina (Verified Customer) (07-31-2023)

antibody worked well for IF of human cardiac ventricular fibroblasts

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human cardiac ventricular fibroblasts

FH

Udesh (Verified Customer) (12-06-2022)

The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

TGF Beta 1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:21898-1-AP)

FH

Yasuyo (Verified Customer) (05-24-2022)

Works great!!!

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: lung

TGF Beta 1 Antibody Immunohistochemistry validation (1:250 dilution) in lung (Cat no:21898-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

TGF Beta 1 Antibody Western Blot validation ( dilution) in fibroblast (Cat no:21898-1-AP)

FH

Iram (Verified Customer) (09-04-2020)

Very clean bands for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer

FH

Qingyuan (Verified Customer) (11-05-2019)

The antibody clearly shows one band with the right molecular weight size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse spinal cord