TGF beta 1 Rekombinanter Antikörper

TGF beta 1 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250119E2

Kat-Nr. : 98506-5-PBS

Synonyme

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Latency-associated peptide, TGF beta

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  FC experiment of human PBMCs using 98506-5-RR (same clone as 98506-5-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TGF beta 1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then fixed and stained with Anti-Human Foxp3. CD4+ cells were pre-gated for analysis. This data was developed using the same antibody clone with 98506-5-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98506-5-RR (same clone as 98506-5-PBS)

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TGFB1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TGFB1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). CD4+ cells were pre-gated for analysis. Cells were not fixed. This data was developed using the same antibody clone with 98506-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98506-5-RR

  Biolayer interferometry (BLl) kinetic assays of 98506-5-RR against Human TGF beta 1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98506-5-PBS bindet in FC TGF beta 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGF beta 1 fusion protein Eg0879
• Vollständiger Name: transforming growth factor, beta 1
• Berechnetes Molekulargewicht: 44 kDa
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.