TGF beta 1 Rekombinanter Antikörper

TGF beta 1 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250119E2

Kat-Nr. : 98506-5-RR

Synonyme

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Latency-associated peptide, TGF beta

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  FC experiment of human PBMCs using 98506-5-RR

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TGF beta 1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then fixed and stained with Anti-Human Foxp3. CD4+ cells were pre-gated for analysis.

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  FC experiment of human PBMCs using 98506-5-RR

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TGFB1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TGFB1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). CD4+ cells were pre-gated for analysis. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98506-5-RR

  Biolayer interferometry (BLl) kinetic assays of 98506-5-RR against Human TGF beta 1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98506-5-RR bindet in FC TGF beta 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGF beta 1 fusion protein Eg0879
• Vollständiger Name: transforming growth factor, beta 1
• Berechnetes Molekulargewicht: 44 kDa
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.