TGFB2/TGF-beta 2 Polyklonaler Antikörper

TGFB2/TGF-beta 2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 28426-1-AP

Synonyme

TGFB2, TGF-beta 2, Glioblastoma-derived T-cell suppressor factor, G-TSF, Latency-associated peptide

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  WB analysis using 28426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28426-1-AP (TGFB2/TGF-beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28426-1-AP (TGF beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 28426-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28426-1-AP (TGF beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28426-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28426-1-AP (TGF beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TGF beta 2 antibody (28426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mausnierengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

28426-1-AP bindet in WB, IHC, IF/ICC, ELISA TGFB2/TGF-beta 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGFB2/TGF-beta 2 fusion protein Ag29126
• Vollständiger Name: transforming growth factor, beta 2
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa, 51 kDa
• GenBank-Zugangsnummer: NM_001135599
• Gene symbol: TGFB2
• Gene ID (NCBI): 7042
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.

Publikationen

Species	Application	Title
human	WB	Front Microbiol; Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma.; Authors - Yang Chen
rat	IF	Front Chem; ADSCs Promote Tenocyte Proliferation by Reducing the Methylation Level of lncRNA Morf4l1 in Tendon Injury.; Authors - Haibo Zhao
human	WB	Life (Basel); MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion; Authors - Zhaodong Li
human	IF	Cancers (Basel); Profiling the Adrenergic System in Breast Cancer and the Development of Metastasis; Authors - Daniela M. Sousa
mouse	WB	J Ocul Pharmacol Ther; Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function; Authors - Xiaochen Wang
rabbit	WB	Small; MiR-141-3p-Functionalized Exosomes Loaded in Dissolvable Microneedle Arrays for Hypertrophic Scar Treatment; Authors - Sheng Meng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (08-14-2025)

Unfortunately, this antibody did not work in our hands. We attempted to use it for western blot on mouse anterior segment tissue (eyes injected with vector expressing TGFb2).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse anterior segment