TGF Beta 1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TGF Beta 1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230544B7

Kat-Nr. : 81746-2-PBS

Synonyme

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

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  WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

  WB result of TGF Beta 1 antibody (81746-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF Beta 1 transfected HepG2 cells. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81746-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

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Geprüfte Anwendungen

Produktinformation

81746-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA TGF Beta 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGF Beta 1 fusion protein Ag13591
• Vollständiger Name: transforming growth factor, beta 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000125
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.