TGF Beta 1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TGF Beta 1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

230544B7

Kat-Nr. : 81746-2-RR

Synonyme

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

---

• 

  WB analysis using 81746-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 81746-2-RR

  WB result of TGF Beta 1 antibody (81746-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF Beta 1 transfected HepG2 cells.

• 

  WB analysis using 81746-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 81746-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 81746-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 81746-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 81746-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 81746-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HEK-293 using 81746-2-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 81746-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, C6-Zellen, HepG2-Zellen, HL-60-Zellen, K-562-Zellen, L-929-Zellen, Mausmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

81746-2-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA TGF Beta 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGF Beta 1 fusion protein Ag13591
• Vollständiger Name: transforming growth factor, beta 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000125
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.

Publikationen

Species	Application	Title
mouse	WB	Free Radic Biol Med; N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3; Authors - Wei Meng
rabbit	IHC	J Orthop Surg Res; Regeneration process of severed rabbit common calcanean tendons influenced by external compression; Authors - Helin Wu
	WB	Life (Basel); Anticancer Effects of Weizmannia coagulans MZY531 Postbiotics in CT26 Colorectal Tumor-Bearing Mice by Regulating Apoptosis and Autophagy; Authors - Bao Zhong
rat	WB,IHC	J Ethnopharmacol; New idea of Fuke Qianjin capsule in treating sequelae of pelvic inflammatory disease: anti-inflammatory in the early stage and reparative in the later stage; Authors - Chunfang Xu
mouse	WB	Front Immunol; Acute hypoxia modulate macrophage phenotype accompanied with transcriptome re-programming and metabolic re-modeling; Authors - Binda Sun
mouse,human	WB	Urolithiasis; Inhibition of NLRP3 alleviates calcium oxalate crystal-induced renal fibrosis and crystal adhesion; Authors - Yuexian Xu